FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_56_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_56_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215418
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22550.18553287840068192No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20830.17138136838519752No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA17480.14381883434341108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA31750.089.059074
AAACACG13600.084.382025
GCACGCA7300.081.450182
TAGTAAC35800.079.75972
AGCACGC7400.079.41521
TAACAAG36150.078.5984955
CGCACTT7850.076.617915
ACGCACT8800.073.0632254
AGTAACA40500.070.5036243
GACGAAA5700.066.49777145
CACGCAC9000.066.065153
GAAACAC17450.065.767494
ACGAGCG852.5102054E-1065.28329
AACACGA13100.064.377416
GCCGACC1700.061.9342145
GTAGTAA48100.059.6512371
ACAAGAG48500.058.436897
CAAGAGC40200.057.5970088
TAGAAAC24400.057.5204962
ACACGAG14850.056.7908447
Produced by FastQC (version 0.11.5)