FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_53_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_53_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587185
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA6810.11597707707111046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC10750.082.96572
GTACTCG250.001133251682.965691
GTAGTAA11600.079.86641
CGACGAA2800.065.18801145
AGTAACA14500.061.509043
TAACAAG14350.060.7066045
GTAACAA14900.058.4657554
AAACACG5950.056.9372375
TGACGCC1750.056.16198145
GCCGACC2050.054.79217145
AACACGA4000.053.5728846
CACGAGC4150.051.8218738
ACGAGCC1900.051.144249
ACACGAG4350.049.2624247
ACGATAA1800.046.801647145
CAAGAGC17450.046.5146688
CGATATT952.3958219E-644.338406145
ACAAGAG20950.041.9047247
AACAAGA21150.041.1816256
GTCCCGA1055.2954165E-639.507471
Produced by FastQC (version 0.11.5)