FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_51_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_51_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412960
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG87020.2549692935164784No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA82420.24149125685621864No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA80310.2353089400403169No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG67060.1964863344428297No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC63640.18646570718672356No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA61650.1806349913271764No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG60740.1779686840748207No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT57950.1697939618395762No Hit
CATATGACCAGAGTGGAAGACTTGTTGCTAAACTTGTTGCTACTGATGAT57630.16885635928929726No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC56400.1652524494866626No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA55530.16270334255309174No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT53020.15534902254934133No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC50390.14764310158923633No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT50240.14720360039379307No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC50110.14682269935774225No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG45450.13316886221930524No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG44730.13105925648117764No Hit
ATATGTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT43800.12833434906942945No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT42850.12555084149828888No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGGTTGACA42440.12434953823074399No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAACATGACCACAACAC42020.12311893488350287No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT41660.12206413201443908No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG41380.12124372978294501No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG39670.11623341615489194No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA38810.11371360930101732No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAA38230.11201420467863672No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC37950.11119380244714266No Hit
GTTTAGGCGGTCTTGACCAGGATAGTCAAGGGCCCTTTGCCATGAAAGCC37590.11013899957807885No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC37530.10996319909990154No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT37320.109347897426281No Hit
GTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACTAGTC36960.1082930945572172No Hit
TTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGGGAGTCCTATCCCAAT36510.10697459097088743No Hit
CAATTGGACCGATTACCCTTCAACACCAGGGAGGTGCCTTGATGACATAG36350.10650578969574798No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA35940.10530448642820309No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA35650.1044547841170128No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT35060.10272607941493601No Hit
AATTGGGATAGGACTCCCACCGCAGTTTCAGCTGCTCGAATTGGCTTTGA34610.10140757582860625No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAA34580.1013196755895176No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA34470.10099737471285922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC10550.076.1879658
CAACGCA11300.074.8588269
GTCCGTC300.002198015370.21792145
GCCGACC4350.067.796616145
GCTGCCC10150.064.3376145
GAACGCC702.9144212E-760.18679145
GTCGCCC8000.057.052063145
TCTGACC10400.056.714474145
GACGAAA27450.056.276657145
TGACGCC10850.053.71509145
AGACGCC1450.053.26877145
CGCCGGG400.00686825252.66344145
GCACGCA3550.052.5771062
ACGTGCA2450.050.941079
AACAACG16400.050.567497
AGCACGC4400.050.275751
GTAACAA69050.047.743494
TATCGGG1204.811227E-946.811947145
TAGTAAC71700.046.4717672
GAAGCGG6200.045.7144431
Produced by FastQC (version 0.11.5)