FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_50_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_50_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079397
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA38430.3560321179325123No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC28290.2620907784624193No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC22740.21067318141517904No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT22110.20483658931792476No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT18560.17194785607149177No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA18180.16842737194933838No Hit
CCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA18160.1682420833113303No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA18150.16814943899232626No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA18110.16777886171631012No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA16090.14906470927749477No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGTTCCCCC15620.14471042628430503No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA15580.1443398490082889No Hit
GATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGT15570.14424720468928484No Hit
GATATAACCCCAGCACATGGTCCCGTGAAGAAAATGGATTATGATGCAGT13970.129424113648639No Hit
GTAGAAACACGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC13750.12738593863055023No Hit
TTCCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCA13670.1266447840785179No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC13650.12645949544050983No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT13100.12136405789528783No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA12220.11321135782293261No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA12180.11284078054691647No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC11740.10876443051073886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG7850.078.354375
AACACGT2500.074.635686
ACGAGCA2400.072.240939
ACACGAG6150.069.668957
GAAACAC8850.069.503994
CAACGCA2900.069.3512959
CACGAGC6450.066.6353768
AACACGA6600.064.918816
TAACAAG13000.063.7941325
GCCGACC3950.062.19656145
TAGTAAC13750.061.3310622
ACGAGCG801.1485099E-860.682389
TCGCGCC350.00405387460.165653145
TAAGTCG350.00431133159.237415
ACAACGC3400.059.1278348
ACGAGCC4000.058.94869
ACACGTG3350.055.698277
GCACGCA1209.640644E-1151.8423422
GTCGCCC3600.050.69513145
GTAACAA17050.048.6429444
Produced by FastQC (version 0.11.5)