FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_4_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_4_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8013494
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA204710.2554566085655022No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG185130.23102282225456214No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA173490.2164973231401933No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT165920.20705075713540183No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG139670.17429351042129687No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG129140.16115317488226732No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT127690.15934372696853583No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT127610.15924389535950234No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC126420.1577589001751296No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA124890.15584962065236463No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT124470.15532550470493894No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG122710.15312920930620275No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA118620.14802531829436696No Hit
CATATGACCAGAGTGGAAAACTTGTTGCTAAACTTGTTGCTACTGATGAT117140.1461784335272479No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC116620.14552952806853042No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG115960.14470591729400434No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT114730.14317100630511484No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC114540.14293390623366037No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA112590.1405005107634697No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC112520.14041315810556543No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC112490.1403757212521779No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA111400.13901551557909697No Hit
ATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT98790.12327955820519737No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGTCAAGAATAAA96740.12072137322371489No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA90490.11292202876797561No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT89960.11226064435812892No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTTTTCCACTCTGG88780.11078812812488534No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG87400.10906603286905811No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT87150.10875405909082855No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC86560.10801780097420677No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA86080.10741881132000598No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA85310.1064579320830589No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT84980.10604612669579587No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT83800.10457361046255229No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC83740.1044987367557772No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA82250.10263937303752896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC21950.097.00671
GCACGCA22250.095.388042
CACGCAC23450.091.087863
GGCACCC4450.075.72942145
ACAACGC23900.075.692798
GAAACAC23750.075.671594
GTAACAA123800.074.3157044
CAACGCA25050.072.536789
AAACACG24900.072.452985
GCTGCCC19100.068.737175145
ACACGTG6750.068.6025167
GCCGACC12100.067.88657145
AACACGT7200.065.274376
TGACGCC22300.064.54051145
CGCACCC2900.062.94464145
TAACAAG147350.062.6716845
ACACGAG21800.062.1396147
TCGCGCC1052.1827873E-1160.17785145
AACACGA23600.057.3997966
TCTCGGG17700.056.721302145
Produced by FastQC (version 0.11.5)