FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_49_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_49_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729452
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30160.17439050057474853No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29310.16947564893388195No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT28770.1663532725973314No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG25730.14877545025823208No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25410.14692515316990584No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22900.13241188538334686No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA22030.12738139017445987No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC21150.12229307318156271No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20250.11708911262064516No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT18640.10777980539500374No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18230.10540911225058573No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG17650.10205544877799441No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC17560.10153505272190266No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA17560.10153505272190266No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA17510.10124594380185169No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT17510.10124594380185169No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC17510.10124594380185169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1900.0109.086362
AGCACGC1900.0109.086361
CACGCAC2000.0103.6260453
CGTTTTT451.3666067E-893.595184145
GAGTCGA250.001067254884.235664145
GGCGCCC250.001067254884.235664145
GAAACAC8950.077.182174
CGCACCC555.4993507E-876.57788145
AAACACG9200.075.833485
GCTGCCC2950.071.38616145
TGACGCC3450.071.21373145
GACGCCC300.00220050370.19639145
GTAACAA33850.069.996364
ACACGAG8250.069.4926767
AACACGA8450.067.847886
ACGAGCC3500.065.395459
TACACAA2600.064.79667145
GACGAAA8100.063.263412145
GCCGACC1700.061.937996145
CACGAGC9050.061.2707378
Produced by FastQC (version 0.11.5)