FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_48_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_48_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129022
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45200.40034649457672217No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37900.3356887642579153No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT33270.2946798202337953No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23250.2059304424537343No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA22750.20150183078806258No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21110.1869759845246594No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA20670.1830788062588683No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT19170.1697929712618532No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT13020.11532104777409122No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG12840.1137267475744494No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12290.10885527474221052No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG11810.10460380754316569No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC11650.10318665181015071No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC11510.10194664054376265No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT11470.10159235161050892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC7050.0117.606651
GCACGCA7200.0115.15652
CACGCAC7900.0105.827363
CGCACTT9600.089.238385
GTAACAA25550.087.614224
GACGAAA4550.086.39715145
ACGCACT10100.084.82444
GAAACAC7450.083.465444
AAACACG7550.081.441225
TAACAAG29600.076.3233265
CGGAGCG503.0711108E-669.0877765
TAGTAAC33900.067.259552
ACAACGC2700.066.756638
AGCGTTT1250.066.551228
GCCGACC959.094947E-1266.503075145
ACGTGCA3300.064.9063952
AGTAACA36750.061.667483
TACACTA451.5154909E-461.4113585
CAACGCA3050.059.130199
AACACGA8700.058.7643176
Produced by FastQC (version 0.11.5)