FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_46_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_46_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA21660.17976550809484249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC20400.073.4901662
GCCGACC3000.070.22738145
ACGAGCG408.307387E-569.343729
AACACGT4650.066.83676
GTAGTAA23400.065.544581
AAACACG13900.065.0977555
ACGAGCC5200.064.009589
CACGAGC9350.063.7388958
AACACGA9800.060.60776
ACACGAG9900.059.9955027
AGCACGC1500.059.8758541
CAACGCA7300.056.044929
AGTAACA26900.055.9868243
TAACAAG26350.055.835255
GAAACAC17200.053.0140574
TTACTCG400.00723498151.9731378
TGACGCC4200.051.83449145
GTAACAA28800.051.329484
GAAGCGG7900.050.7224851
ACAACGC7050.049.1471758
Produced by FastQC (version 0.11.5)