FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_42_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_42_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079572
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23930.22166191787115633No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22210.2057296780576006No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21300.1973004116446147No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19070.17664407746773722No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19070.17664407746773722No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC13220.12245593624139936No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA13160.12190016043394974No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT12910.11958442790290967No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12760.11819498838428562No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC12670.1173613246731112No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA12580.11652766096193676No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12510.11587925585324554No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT12340.11430455773213828No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC12310.11402666982841347No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG12230.11328563541848065No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT12130.11235934240606461No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT12040.11152567869489019No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11710.10846891175391729No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA11540.10689421363281003No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11530.10680158433156843No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC11280.10448585180052834No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11210.10383744669183713No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG11210.10383744669183713No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG11070.10254063647445469No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC10830.10031753324465621No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT10810.100132274642173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA151.4001061E-4140.3612145
GTAACAA17200.0110.1163944
GAGCCCC451.09139364E-10109.16982145
AGCACGC1600.0103.691031
AAACACG5850.0100.432165
GAAACAC6100.096.320554
CACGCAC1750.094.803243
GCACGCA1750.094.803242
TAACAAG21050.090.300535
CGCACCC407.424005E-787.72575145
AACACGT1950.085.071946
CTTCCGC250.001068097884.21673145
TCTAGGG250.001068097884.21673145
AGTAGCC250.001068097884.21673145
GAAGCGG2000.079.496461
TCTACTA652.2828317E-974.437955
ACACGTG2250.073.729027
GAGCAGA1700.073.193671
ACGTGCA2650.073.040222
TAGTAAC26100.072.570482
Produced by FastQC (version 0.11.5)