FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_40_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_40_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936702
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25970.277249327961294No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25900.2765020251905088No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22650.24180582511834073No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22520.240417977115454No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19490.208070442894325No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA15010.1602430655640748No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC14700.15693358186488338No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13890.14828622123151228No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT13860.14796594861546147No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC13800.14732540338335992No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG12720.13579558920553175No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT12550.13398071104791065No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG12540.13387395350922704No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA12400.13237934796765674No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA12190.13013743965530125No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC11670.12458604764375436No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA11640.12426577502770358No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC11620.1240522599503364No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA11320.12084953378982857No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC11290.12052926117377778No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT11230.11988871594167622No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG10380.11081432515357072No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA10060.1073980839156957No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT9970.10643726606754336No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG9800.10462238790992226No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT9520.10163317682678162No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC9380.10013857128521131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA19600.0107.5521854
GAAACAC6050.0103.958864
AAACACG6200.0102.558495
GTCGCCC555.456968E-12102.14028145
CACGCAC1150.096.181243
TAACAAG22800.092.760285
GCACGCA1200.092.173692
AGCACGC1250.088.486741
GCTGCCC1450.087.17145145
AACACGA5950.083.622186
ACACGAG6300.077.87967
TAGTAAC29200.073.628462
AGAAGCG1250.071.880125
AACACGT1950.070.876346
TCGCCGC502.7877595E-670.22144145
CGGAGCA5350.068.4841842
ACAAGAG30550.068.312737
TAGAAAC10450.067.476432
AATGGCC1050.066.87757145
GAGCAGA3350.066.034881
Produced by FastQC (version 0.11.5)