FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_39_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_39_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755168
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTAAAAACAATTTTAGGTAGAAAACAGAGTGTGAGTT13670.18101932285266323No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG10140.1342747574049748No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC9180.12156235433704818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG250.001064948384.276825145
GTAGTAA11150.084.2724841
TAGTAAC11700.079.720432
CGGAGCG950.072.7081455
ACACGAG5100.065.005337
AGTAACA14350.064.989933
ACACTCG350.004044845260.19774145
ACTCGAT350.00432125659.2012837
ACAACGC3900.056.8444488
AACACGA5900.056.191056
CACGAGC5950.055.8890848
AAACACG9550.055.6921545
AACACGT4100.053.906866
ACGAGCC2350.053.0966389
GCCGACC2400.052.67302145
AACAACG4750.049.4382677
TGACGCC3750.048.693283145
GTAACAA19300.047.241464
ACGAGCA2400.046.2137379
TAACAAG19750.046.165075
Produced by FastQC (version 0.11.5)