FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_38_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_38_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353967
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29660.2190599918609538No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26110.19284074131791987No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22460.165882920337054No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT21780.1608606413597968No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19320.14269180858913105No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA17450.12888054140167374No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA15700.11595555873961479No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC15440.11403527560125172No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC15110.11159799315640634No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT15040.11108099384992397No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG14950.11041628045587522No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC14670.10834828322994579No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA14540.10738814166076426No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT14530.10731428461698107No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT14430.10657571417914911No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA14210.10495085921591885No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC13910.10273514790242304No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA13880.10251357677107345No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT13840.10221814859594067No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT13730.10140572111432553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC2650.0109.550691
GTAACAA19650.0109.026144
GCACGCA2950.098.4099352
CACGCAC3000.096.7662053
AAACACG6900.093.14285
TAACAAG24050.089.6509555
GAAACAC7250.089.6027454
GTCTCGC250.001134668482.945521
GAAGCGG3100.078.040141
CGGAGCG652.2882887E-974.421795
TAGTAAC29800.072.832472
CTCGCTC300.002339634669.118713
ACGCACT4600.067.606154
CGCACTT4600.067.6036455
TGACGCC3900.066.632805145
GCCGACC2750.066.4036145
AACACGA7400.066.289666
AACACGT2300.066.086696
TAGAAAC12050.063.098252
ACACGAG7900.062.968687
Produced by FastQC (version 0.11.5)