FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_37_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_37_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614652
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGGGGGTTGGAGGAGTTGAGGGTGAAAGTCCGGGCAA9180.14935280451377364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA7750.083.80511
AAACACG9150.074.752045
ACGACGA300.002200461770.191185145
TAGTAAC9200.069.0946352
ACACGAG6900.062.0698747
CACGAGC6800.061.1549388
TAACAAG11050.057.522075
AACACGA7650.055.9845966
ACGAGCA3750.055.492439
AACACGT4900.052.1607636
ACGAGCC2700.051.3818869
AAGAGCG3450.050.264899
GTAGAAA16300.048.7477461
TAGAAAC16950.047.2862
GTAACAA13550.046.909144
GCCGACC1352.6193447E-1046.79413145
AGTAACA13600.046.7404863
GAAACAC14800.046.2149434
CCGACGC804.5188746E-543.86949145
ACGAGCT1900.043.809829
Produced by FastQC (version 0.11.5)