FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_35_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_35_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948673
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG6350.072.969877
GTAGTAA12600.072.4781
AAACACG10600.070.4703145
ACGAGCG601.08706445E-769.399439
AACACGA7000.066.19416
TAGTAAC14900.061.7544332
ACGAGCT2150.058.1018459
CACGAGC8200.056.6744358
ACGAGCC3250.053.384189
AACACGT5650.047.737396
GTAACAA18850.047.7027244
TAACAAG19000.047.3236275
GCCGACC2700.046.800957145
TGACGCC2850.046.800953145
AGTAACA20150.045.6573453
GTAGAAA23450.043.073761
ACAAGAG22300.040.0062077
TAGAAAC24800.039.892122
CAAGAGC21950.039.816438
AAGAGCG3350.039.360879
Produced by FastQC (version 0.11.5)