FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_32_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_32_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015002
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT30600.3014772384684956No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT25780.2539896473110398No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA22920.22581236293130455No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA21520.21201928666150413No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA20090.1979306444716365No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC19790.194974985270965No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA19670.19379272159069635No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC17900.17635433230673436No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA17200.16945779417183415No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA16490.1624627340635782No Hit
GTTATAATTTTACCTATTTCCCAACTTTTTGTTATTGGATCTCTCCACCA16050.15812776723592661No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA16000.15763515736914804No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA15990.15753663539579232No Hit
GCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTTCGTTGTGGAG15850.15615732776881228No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC15640.15408836632834222No Hit
GCCCAAGCCATTGTTGCGAAGAATCCGTTTCCATTGGTAACGTTAGGGCA15520.15290610264807358No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA15360.15132975107438212No Hit
GAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAACAAG15220.14995044344740208No Hit
CTTCTATTGTTAATGGATTTGTTGCTGTTTTGTTTTTGTCGTTTTTTGGG14720.1450243447796162No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC14020.13812780664471597No Hit
CCCATACACAATTGAATCTATCATTATTAACTTTCAAATTTTTGAGCCTG14010.13802928467136028No Hit
GCTATCATCAGTGTTACAGCCAAACTGGAGGCAGCAGTTGAGTAGTAAAG13460.1326105761367958No Hit
TTCTCATCTAGTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCT13420.13221648824337293No Hit
ATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATATTCAGTGAATCAA12950.12758595549565419No Hit
CTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA12880.12689630168216418No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG12520.12334951064135835No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC12120.11940863170712963No Hit
CTCAAAGAGTTGAGATTTTTTGTTATCTTGTTTATGGCCTCTTGAGTGCT12080.11901454381370677No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA12030.11852193394692818No Hit
GGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAGCTGCAAA11990.1181278460535053No Hit
GTTTATGGCCTCTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCAT11790.11615740658639097No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA11240.1107386980518265No Hit
AATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAAGGTACCAGCAGCT11020.10857121463800071No Hit
ATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCTA10800.10640373122417493No Hit
GATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGG10750.10591112135739633No Hit
GCCCAAAGGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAG10630.10472885767712774No Hit
GTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCTTAGTCTTTGA10440.10285694018336909No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG250.001066853184.24107145
ACGAGCG555.999573E-875.617389
GTAACAA14000.069.5737154
AGCGATC300.002317265169.2850958
ATTAGTA4450.065.218651
TTACGCC3450.065.11388145
CTAGGGT1200.063.3235934
TAACAAG15300.063.2107125
AACACGT2900.061.9248966
ACACGAG3700.061.603047
TAGTAGT3150.061.4167983
GCGTATT350.004052057460.1722145
ACGTGCG350.00426083859.4136479
GCGATCC350.00426083859.4136479
CAGCGAT350.00432121159.202927
CACGAGC3900.058.625858
TAGGGTA1201.8189894E-1257.5669025
GCCGACC2500.056.16072145
AACACGA4200.054.2693446
TGACGCC4300.053.875107145
Produced by FastQC (version 0.11.5)