FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_31_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_31_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773788
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14620.1889406400719577No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC14190.18338356242278248No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA10920.1411239254162639No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT9320.12044642718677467No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC8340.10778145952121253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC3500.0108.720061
GTAACAA20300.0100.5275044
GCACGCA4100.092.809822
TAGTAAC22100.092.664742
TAACAAG23150.088.450375
CACGCAC4550.083.636233
AGTAACA24950.082.08513
GTCTCGC3950.080.570451
AAACACG9500.075.0019455
CTCGCTC4300.074.017183
GAAACAC10350.068.1739964
GCCGACC1350.067.60199145
GTAGTAA31450.067.0956341
ACAAGAG30300.066.8891757
CGCACTT5750.066.1688845
TCTCGCT4850.065.619232
ACGCACT6350.061.0060964
CGCTCTG5150.060.4455385
CAAGAGC27550.060.2100878
AACACGT3150.059.286536
Produced by FastQC (version 0.11.5)