FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_28_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_28_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656096
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20350.31016802419158174No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18000.274350095108033No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT14570.22207116031800225No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12740.19417890064868554No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT11190.17055430912549385No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT10930.1665914744183778No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC10300.15698922108959665No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT10100.1539408866995074No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA9890.15074013558991367No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG9710.14799663463883334No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT9010.13732746427352094No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT8620.1313832122128469No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC8040.12254304248158807No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC7530.11476978978686046No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC7400.11278837243330245No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT7210.10989245476271765No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT7160.10913037116519533No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT7080.10791103740915964No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG7030.10714895381163732No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA7000.10669170365312393No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC7000.10669170365312393No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC6990.10653928693361947No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG6900.1051675364580793No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA6760.10303370238501683No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA6760.10303370238501683No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA6760.10303370238501683No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT6570.10013778471443203No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG6570.10013778471443203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC254.499998E-8140.38618145
GAGCCCC151.3988624E-4140.38618145
ACAGCGT151.4830095E-4138.342454
ACCCGCA100.008161795138.331927
GAAACAC4550.0114.01854
TTCGGAA257.59017E-6112.308945145
AGCACGC1550.0111.5751
GTAACAA10900.0111.0547264
CACGCAC1600.0108.088293
GCACGCA1600.0108.088292
AAACACG4950.0106.202285
CACAGCG204.6586344E-4103.7647553
GAGCAGA800.0103.7647551
AGTAGCC301.8775136E-593.5908145
TAACAAG12950.093.474635
AACACGT1350.092.221276
TAGAAAC6200.091.491492
TACGCGG407.4128366E-787.74137145
CGCACCC250.001067152784.23171145
CAGCGTT250.00113115683.005475
Produced by FastQC (version 0.11.5)