FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_24_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_24_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865305
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA12970.14988934537533008No Hit
GTAGTAACAAGAGCACAAGGGGCAAAGATGACGACAGTTTTGGCTACTTC11090.12816290209810413No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA10900.12596714453285257No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC9560.11048127538844685No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC204.6253286E-4103.954958
GTAGTAA14000.085.411341
TAGTAAC14900.080.2522662
ACAACGC6100.065.8949368
AAACACG10650.064.2328955
GCCGACC2400.061.47059145
GACGTAA350.00404016960.216084145
AATTACG350.004308897559.2448582
AACAACG7000.059.2208947
AGTAACA20450.058.120883
AACACGT6100.056.6320046
GTAACAA21250.055.607614
ACGAGCG1251.8189894E-1255.4747969
ACACGTG6150.052.8012857
ACGTGCC3300.052.532959
AAGAGCG4200.051.1821029
CAACGCA9800.050.238659
TAACAAG23750.049.75135
ACACGAG7550.049.416117
AACACGA7900.048.101366
Produced by FastQC (version 0.11.5)