FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_23_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_23_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985122
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA16830.17084178406329367No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA12940.1313542891134296No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA10130.102829903301317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC21150.084.064212
GTAGTAA22300.079.418831
AAACACG9500.072.07955
GTAACAA25950.067.9677664
AGTAACA26100.067.587443
ACACGAG6950.061.7031147
TAACAAG28750.061.3482935
ACAACGC4200.061.1098188
AACACGA7400.059.820286
CACGAGC7200.054.9162528
CAACGCA4850.051.5184259
CAAGAGC31800.050.8261688
ACAAGAG36550.048.0669447
CGTCGCC605.783393E-446.82208145
AACAACG5500.046.5306637
AACACGT5500.045.2730756
AAGAGCG5250.043.627119
GTAGAAA23650.043.5856481
AACAAGA40750.043.2825436
CGGAGCG659.249538E-442.564435
Produced by FastQC (version 0.11.5)