FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_22_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_22_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633889
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA34340.21017339611197577No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG29670.18159128312878048No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA27870.17057462287829833No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA27250.16677999545868782No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT26280.16084323965703912No Hit
GTATTAGCAGGCTTTCTCGTTAGTGGCAAATATGACCCAGATCTTGGAGA22630.13850390081578368No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG20520.1255899268554963No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA19940.12204011410811871No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT19850.12148928109559462No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG19840.12142807742753639No Hit
GTTCCAATGGGATGCATTTGAAGCATTTGAGAGCATAATTCCTCAGAAGA19210.11757224633986764No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA18690.11438965560083947No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT18550.11353280424802419No Hit
CCAAAGAACTGGTGCAAAACACTTATCAATGGGTGCTAAAAAACTTGGTG18210.11145187953404423No Hit
AAGCAGAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA18030.11035021350899601No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT17970.10998299150064661No Hit
ATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGC17680.10820808512695783No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA17150.10496429071987143No Hit
CATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTG16970.1038626246948232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC20500.079.249462
GTAGTAA22550.075.110711
AAACACG19150.073.2706155
ACACGAG13400.071.176557
CGGAGCG601.11838744E-769.119815
AACACGA13900.068.119026
AGTAACA24600.066.597153
AACACGT7050.064.7019656
ACGAGCT5300.064.103359
GTAACAA25600.063.9898264
CACGAGC15250.061.802068
GAAACAC23350.060.9793624
ACGAGCC2850.060.8212979
TAGAAAC29200.060.372572
ACAACGC5850.056.861628
TAACAAG29550.056.371835
CAACGCA5850.055.7060669
TGACGCC6050.054.55464145
TCACGAA400.007306621451.844623
GTAGAAA36650.049.7980421
Produced by FastQC (version 0.11.5)