FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753197
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20740.2753595672845219No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17580.23340507197984062No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC17430.23141356112677028No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA16500.21906619383773437No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15170.2014081309405109No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT15130.20087706137969216No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14750.19583190055191402No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13910.1846794397747203No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC13650.18122748762939841No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC11990.1591881008554203No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG11660.1548067769786656No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT10660.14153003795819688No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT10460.13887469015410311No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT9950.13210355325366405No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA9760.129580972839775No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA9600.1274566945965No Hit
AAAATGAACAATGCTACCTTCAACTATACAAACGTTAACCCTATTTCTCA9340.12400474245117812No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT9150.12148216203728905No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG8810.11696807077032968No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA8680.11524209469766873No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT8640.11471102513684998No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT8620.11444549035644061No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT8500.11285228167398435No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC8450.11218844472296094No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8370.11112630560132343No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC8180.10860372518743436No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC8050.10687774911477342No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC7950.10555007521272654No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT7820.10382409914006561No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT7710.10236365784781405No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC7710.10236365784781405No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG7680.1019653556772No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG7650.10156705350658592No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA7650.10156705350658592No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA7540.10010661221433437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG100.0077853096140.5359145
GTAACAA17550.0101.122914
GGATCCC353.326477E-7100.382805145
TAACAAG19750.089.858595
GAAACAC4100.085.897384
GCCGACC1250.078.70011145
TACGCGG451.484439E-678.07551145
AACACGT1350.076.707126
AAACACG4600.076.56075
TAGTAAC25550.070.289652
GAAGCGG2100.069.07311
AGCACGC408.467764E-569.07311
ACACGTG1450.066.6558467
TGACGCC1900.066.56964145
AGTAACA27500.065.049983
AACACGA4350.063.4817626
ACAAGAG28150.063.0279127
GCACGCA555.412143E-662.7937242
CACGCAC555.4142765E-662.789553
ACACGAG4550.060.6913537
Produced by FastQC (version 0.11.5)