FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_16_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_16_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794709
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21530.27091677582612No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19040.23958455233299233No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT17100.21517310109738283No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG14160.17817842757537664No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA13890.17478095755804954No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC13420.16886684308344313No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT11180.14068042516191462No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA10050.12646138397828638No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA9940.12507722952678277No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG9080.11425565836048163No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA8950.11261983946325006No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT8810.11085818834315453No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA8660.10897070500019504No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG8070.10154660385122101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC4050.0102.558061
GCACGCA4500.092.3022462
CACGCAC4850.087.0576553
GTAACAA18350.081.094974
AAACACG6950.078.674485
ACAACGC2100.075.963128
GAAACAC7400.074.825534
GCCGACC1250.072.95532145
TAACAAG20650.072.062615
CGCACTT5750.071.019195
ATATACG300.002326004469.217983
ACGCACT6200.069.2136154
AACACGA5850.066.25166
TAGTAAC22600.066.163552
ACACGAG6000.064.595317
CGTGCCC451.5011725E-461.527093
AGTAACA24350.061.4007453
AACACGT2600.061.2235766
GTAGTAA25700.059.529561
ACGATCC1350.057.158733145
Produced by FastQC (version 0.11.5)