FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_15_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_15_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021648
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT56420.5522450002349146No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT50760.4968443142843719No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT43960.43028518628725354No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT42190.41296023679388594No Hit
GAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC37870.370675614301599No Hit
CACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA33860.33142530499741596No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA33210.3250630354094561No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT32810.3211477925860962No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC32400.3171346686921523No Hit
AAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACA32180.31498128513930435No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT27500.26917294410599346No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT27220.2664322741296415No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26300.25742721563591375No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT23160.22669255947253847No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG22770.2228751977197626No Hit
CTCTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT22370.21895995489640266No Hit
AGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA19680.19262994690930732No Hit
GGATGAAGTGGGCCATGTGTTCTAACTTTCCCTTGGCTCTACCCAGATCT19050.18646343946251545No Hit
CATCTTGCTTTATCAAGGAAAACCAGTTAAAGTAGTGAAAAGGAAAAGGT18620.18225455342740357No Hit
GTATAGTGCTTTGTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAA18470.1807863373686436No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT17710.17334737600425978No Hit
ACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGCTGAAA17620.1724664463690038No Hit
AATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTG17400.17031306281615585No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT16900.16541900928695596No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC16410.16062283682834008No Hit
GAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAAC16360.1601334314754201No Hit
GTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT16020.1568054750755642No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT15940.1560224265108922No Hit
GTTAAAACAACTGCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAA15180.1485834651465084No Hit
AACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATT15140.1481919408641724No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT15090.1477025355112524No Hit
GCTAAGGGACAATGAGGCCAAAACAGTTTTGAAGCAAACAACAGTAGACC14830.14515762767606846No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14770.1445703412525645No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG14340.14036145521745258No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTT14060.13762078524110066No Hit
GAATTAAGAGACAAAGAATGACAGTTGAGTCTATGGGGTGGGCCTTGAGC13840.1354674016882527No Hit
GCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTC13750.13458647205299673No Hit
ACTTTAACTGGTTTTCCTTGATAAAGCAAGATGTTTGCCTTTTCCCCTGG13540.13253096957073277No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT13510.13223732635898078No Hit
TCTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTT13450.1316500399354768No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA13310.13027970494730082No Hit
GTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT13220.12939877531204486No Hit
ATTCAATACATCAAGGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGG13200.12920301317087687No Hit
CTTAATTCCTTGTGAAATGTCATTGGACAAAGCACTATACCTTTTCCTTT12890.1261686999827729No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA12490.12225345715941302No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT12030.11775092791254914No Hit
GCTCTACCCAGATCTTGGAGATTTCAAAACTATTGAAGAACTTGAAAAGC11940.11686999827729316No Hit
GACCAATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAA11390.11148653939517328No Hit
GAATTAATGGATAAATTTATATTAGCTCAAGGCCCACCCCATAGACTCAA11380.11138865832458929No Hit
GTCCAATGACATTTCACAAGGAATTAAGAGACAAAGAATGACAGTTGAGT10770.10541791301896543No Hit
AGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTGTTAAAACAA10710.10483062659546145No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA10610.10385181588962147No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA10520.1029708862543655No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATAGTTTTGAAATCTCCAAGATCTGG10470.1024814809014455No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT10460.10238359983086151No Hit
GTTCTAACTTTCCCTTGGCTCTACCCAGATCTTGGAGATTTCAAAACTAT10430.10208995661910951No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10360.10140478912502153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA254.4939952E-8140.42749145
CGTCAGT100.007803705140.42749145
TTCGTGT100.008174784138.26213
GTAACAA26400.0119.664184
AGCACGC5800.0116.824781
CGGTGCA301.4619582E-7115.235322
CACGCAC5950.0115.0247653
GCACGCA5900.0114.84472
GGCCCCC257.581144E-6112.34199145
TAACAAG28650.0110.266465
GAAACAC12500.0107.2861254
CACAGCG600.0103.696563
ACAGCGT600.0103.691484
AAACACG13300.099.793325
CAGCGTT650.095.7152255
TAGTAAC34900.091.52792
GTAGAAA15500.089.660531
AGTAACA36600.087.452663
CAACGCA2800.084.270749
TAGAAAC16700.082.803832
Produced by FastQC (version 0.11.5)