FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_13_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_13_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840433
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30250.35993351046424876No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG26360.3136478458128132No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA25800.30698461388355763No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT17510.20834498407368585No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC16150.19216284938835101No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG13810.16432005882681902No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG13340.15872770345762244No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA13110.15599101891524964No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA11860.14111773335887573No Hit
TCTCTCTTCTGTCCTTCCAAGCCTACTAAGCACGTGGCATCCTCCCAGAT11490.13671524083418904No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC10690.12719633807810973No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT10660.12683937922475677No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG9530.11339392908179474No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG9090.10815853256595112No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG9070.10792055999704914No Hit
CCTTTATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTA8950.10649272458363726No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC8810.10482691660132337No Hit
GTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAAACAATGTAATT8670.10316110861900947No Hit
ATACAAAGGAGCAGATATGCAGAATGAATAAGTCTAGAAGATCTAGTGTG8500.10113834178334263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG801.3096724E-1070.21282145
GCCGACC1900.070.21282145
GTAGTAA24500.069.4126051
AGCGGTA300.002312471669.320248
TAGTAAC26050.065.017112
ACAACGC6250.061.0018048
AACAACG6500.058.4741067
AAGAGCG5000.056.866359
ACACGTG5650.053.8168757
AGTAACA32050.053.4861453
GTAACAA32450.051.3327034
TGACGCC2500.050.55323145
CAACGCA9250.048.7318769
TAACAAG34750.047.935145
AACACGT6650.047.802486
TTACGCC2250.046.80855145
CAAGAGC34950.045.2217868
GACGCTC1309.753421E-943.20789145
ACAAGAG38850.042.8686947
ACGTGCC3250.042.6764349
Produced by FastQC (version 0.11.5)