FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_12_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_12_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246548
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA41590.33364138404618193No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17540.1407085808167836No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA17330.13902392848089284No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG16970.13613595304793719No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG16300.1307611098810475No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15770.12650936827141834No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT15750.1263489251918097No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA15520.12450382977631025No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG13300.10669464793975042No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC12830.10292423556894721No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG12680.10172091247188234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC28150.090.356122
GTAGTAA28400.089.804111
AAACACG16350.079.8812945
GTAACAA31400.079.667284
ACGAGCC5050.075.5249949
AACACGA11950.074.013046
TAACAAG33800.074.010425
ACACGAG11800.073.2000967
AGTAACA34750.072.791323
GAAACAC18300.072.124594
GCGACCC300.0021975370.21915145
TATCGGG300.0021975370.21915145
AACACGT6400.070.177776
ACGAGCA4850.070.0605859
CACGAGC12250.069.045968
TGACGCC3150.064.646194145
GCCGACC3200.061.44175145
AGCACGC2550.056.920311
TAGAAAC29550.056.6038632
ACAACGC4350.051.0005958
Produced by FastQC (version 0.11.5)