FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_121_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_121_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413516
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT227160.3541894960580125No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC214990.33521394504979796No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT201670.31444530581977187No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG112080.1747559373049042No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA99160.15461098093463865No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA95990.14966829427103637No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT87750.13682042735996916No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC87020.13568220614090618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC50350.0109.281211
GCACGCA51050.0107.511932
CACGCAC56250.098.053133
GTAACAA165650.093.9998554
AAACACG56150.090.342095
CGCACTT66100.084.270875
GAAACAC60500.084.077524
ACGCACT69500.080.647974
TAACAAG196250.079.727955
GTCTCGC17000.076.850161
TAGTAAC205900.076.342442
AGTAACA224650.070.11633
CTCGCTC18650.069.6722953
GTAGTAA235850.068.054721
TGACGCC12450.064.85899145
GCCGACC7800.064.81561145
GAAGCGG18150.064.3638461
ACGTGCA21400.064.279312
ACAACGC19650.062.421558
AACACGT16400.061.5213436
Produced by FastQC (version 0.11.5)