FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_11_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_11_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686734
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT44430.6469753936749891No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC42180.6142116161424948No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA27610.4020479545209644No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA21240.309290059906747No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19950.2905054941214502No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT18820.27405079696068635No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT16760.24405373841982486No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG15580.22687095731389445No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT13060.19017552647750074No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG12790.18624387317360144No Hit
AGTAGAAACAACGCACTTTTTCCCAGTTTATTTGCTGACATTGATTACAA12150.1769243986754697No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA11830.17226466142640381No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT11020.16046970151470585No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT10560.15377132921917366No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA10150.147801040868808No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG10050.14634487297847493No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG9820.14299568683070885No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA9740.14183075251844238No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA9730.14168513572940905No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG8920.1298901758177111No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT8490.12362865388927881No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT8490.12362865388927881No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT8430.12275495315507896No Hit
GTAAGAAGTACAGCAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA8300.12086193489764596No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT8270.12042508453054604No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC8180.11911453342924626No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG8110.1180952159060131No Hit
GAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTTAAAAC8020.11678466480471332No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC7730.11256177792274738No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT7540.1097950589311145No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGATGCTAGCTA7390.10761080709561491No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG6930.1009124348000827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCC100.007794817140.47765145
AGCACGC9350.0120.565991
CACGCAC9350.0120.5484163
GCACGCA9300.0120.470542
GAAACAC6150.0111.3129354
GCGGTGC258.171581E-6110.654421
AAACACG6450.0106.127865
GTAACAA20100.0103.2073754
CGCACTT12750.091.107265
ACGCACT12900.090.0544364
TAGTAAC24250.086.9835052
AACACGT1950.085.081596
AGTAACA25350.083.469733
TAACAAG25150.083.027675
CGGAAAA354.0211904E-580.27294145
AGACGCC354.0211904E-580.27294145
GTAGTAA26500.079.8590851
ACAACGC3300.077.68918
GAAGCGG1200.074.9222641
AACACGA7150.072.512726
Produced by FastQC (version 0.11.5)