FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_117_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_117_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151641
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT67000.21258766464835305No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC57470.18234944906478878No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC42390.1345013597678162No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT40060.12710838575840333No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG40050.127076656256217No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC39340.12482386160098818No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA38820.12317392748729947No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT38360.12171437038672869No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA38230.12130188685830652No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT38170.12111150984518858No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC36710.11647900252598567No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG35260.11187822470896908No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA35100.11137055267398793No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA34290.1088004629968959No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG33840.10737263539851144No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33830.10734090589632511No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA33440.1061034553110586No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC33260.1055323242717048No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33230.10543713576514585No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC33150.10518329974765527No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA31700.10058252193063867No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA31590.10023349740658914No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT31540.10007484989565754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC555.456968E-12102.08549145
GTAACAA47100.098.1581654
GAGCAGA6100.095.180171
GAAACAC15050.087.703734
AAACACG15400.086.1578455
TATCGGG250.001068100384.220535145
TAACAAG61100.076.118145
ACACGAG13950.074.794367
AACACGA14550.071.710056
AGACGGG300.002202243370.18377145
ACACGTG4200.069.09817
AACACGT4200.069.09816
CACGAGC15150.067.706958
TAGTAAC70900.066.876712
GCTGCCC9250.063.73445145
AACGGCC451.402369E-462.385582145
GCCGACC4400.062.208347145
TCTCGGG6800.061.926857145
GTCGCCC4050.060.65265145
CAACGCA4850.058.6304939
Produced by FastQC (version 0.11.5)