FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_110_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_110_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6135362
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA66140.10780130006998773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC25400.064.954318
AACACGT21700.063.0658266
GCCGACC10250.061.635513145
AACACGA34200.060.4273726
TAGTAAC116600.060.3063772
AAACACG58000.059.8307655
GTAGTAA121250.059.475741
ACACGAG35450.057.9081237
CAACGCA32250.056.9940389
AACAACG30050.055.4334377
TGACGCC17600.055.04005145
CACGAGC37300.054.267378
CGACGAA21600.051.997044145
GTAACAA142450.048.7707564
AGTAACA144200.048.5671423
ACGTGCA16600.046.3797239
TAACAAG153500.045.4392975
TCTCGGG29650.045.219013145
ACGAGCA26700.042.863349
GAAACAC83150.041.9009784
Produced by FastQC (version 0.11.5)