FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_10_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_10_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463337
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26790.18307471211347762No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA24970.17063738564664188No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23030.1573800156764983No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22850.15614995042153654No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22360.15280143944969615No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA21500.14692446100932322No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT20740.14173085215504017No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG19870.13578553675605826No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18590.12703840605410782No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC17860.12204980807565176No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT17640.12054639498625401No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT16990.11610449267666983No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC16680.11398604695979121No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG16480.11261930778761145No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT16110.11009084031907893No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC15980.10920245985716209No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG15840.10824574243663626No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC15840.10824574243663626No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15770.10776738372637334No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA15510.10599062280253968No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT15120.10332548141678917No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA15110.10325714445818017No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT15090.1031204705409622No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG15010.10257377487209031No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA15000.1025054379134813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCA100.0078090634140.39734145
GCGCAAC100.0078090634140.39734145
AAACACG5750.097.401575
GAAACAC5850.095.736594
GTAACAA20800.093.742074
GCACGCA556.0572347E-1088.006312
AGCACGC556.0572347E-1088.006311
TTCGGAA502.8387149E-884.23841145
GAGCAGA2150.080.404441
TAACAAG24550.079.423035
AACACGA6000.076.049616
GCCGACC2500.073.00662145
ACACGAG6300.072.42827
AACACGT1450.071.5200046
TAGTAAC29200.068.200592
GAAGCGG3250.068.0841
GCTGCCC4050.065.865425145
CACGCAC757.063136E-964.535763
ACGTGCA2550.062.3686142
TCGATCC350.004052929660.17029145
Produced by FastQC (version 0.11.5)