FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_109_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_109_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019829
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC40550.080.1204762
GTAGTAA42800.078.654151
AAACACG33300.063.9229245
AGTAACA55000.058.943983
AGCACGC12600.055.4098931
AACACGA25100.055.339676
CGACGAA8450.053.999638145
ACACGAG25600.053.719827
ACGAGCG2200.053.5906839
CACGAGC26750.051.0483638
TAACAAG67900.048.3474965
GTAACAA67900.048.247314
AACACGT15250.048.034176
ACGAGCA11500.047.039199
GCACGCA15100.045.7782862
TGACGCC11150.044.70105145
GAAGCGG17550.042.932471
AAGCGGA17050.042.5697822
GCCGACC6150.042.233868145
GTAGAAA74900.041.622791
Produced by FastQC (version 0.11.5)