FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_105_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_105_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280630
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT39890.3114873148372286No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA30650.23933532714367148No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28100.21942325261785217No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA24450.19092165574756176No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC24310.1898284438128109No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT23130.18061422893419643No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT22200.17335217822478No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT21650.16905741705254446No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT21070.16452839618000514No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA20820.16257623201080718No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT20710.16171727977636008No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19730.154064796233104No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA19610.153127757431889No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA18720.14617805298954423No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATG18640.14555336045540085No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT17880.13961878138103903No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA17610.1375104440783052No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC17230.13454315454112428No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT16970.1325129038051584No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT16460.12853048889999455No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC16300.12728110383170782No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA16190.12642215159726072No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15680.12243973669209686No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA15640.12212739042502518No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC15250.11908201432107636No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA15060.11759836955248588No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT13630.10643199050467347No Hit
ATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCAT13110.10237148903274171No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA13020.10166870993183043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA100.0078009507140.44548145
GCGAGGA100.0081711495138.284032
AGCACGC2400.0103.713011
CACGCAC2600.095.7313463
GCACGCA2650.093.928762
GCCGACC1600.092.16734145
GAAACAC9150.090.663884
AAGAGCC7400.088.08719
GTCGCCC1600.087.77842145
AAACACG9700.085.523145
GAGCAGA3650.083.349271
TGTAGCG250.001134006982.957445
GTAACAA27200.079.2975544
AACACGA9450.070.946226
CAACGCA2750.070.605989
TAACAAG30450.070.379885
GCTGCCC3000.070.22274145
AACGGCC300.00219709870.22274145
ACACGAG9750.068.763257
AACACGT2400.066.23786
Produced by FastQC (version 0.11.5)