FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_104_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_104_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624502
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC31100.19144328538838365No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA26530.1633115871817948No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG25870.15924880363335964No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA25630.15777142779756503No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT23220.1429361121131276No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT23170.1426283254806704No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT22800.14035070440048705No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG20610.12686964989886132No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC20180.12422268485972933No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT19850.12219129308551176No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC19240.1184362961695338No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT18800.11572777380391037No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC18620.11461974192706441No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT18260.11240367817337252No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC18100.11141876094950946No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17240.10612483087124545No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA17190.10581704423878827No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA16960.1044012257294851No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA16790.10335475117913058No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG16710.10286229256719906No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC16710.10286229256719906No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT16280.10021532752806707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAG33600.083.738085
GTAACAA34350.081.9097444
GCCGACC2700.080.56967145
AACACGT3300.075.4054956
AAACACG10400.074.447925
TCTCGGG5400.072.772606145
AGACGCC502.800014E-670.173584145
CGTCATA300.002203316470.173584145
TAGTAAC40700.069.310742
ACGAGCC4100.067.6941769
GAAACAC11550.067.035364
AACACGA7950.065.209156
ACACGAG8000.064.803597
CACGAGC7950.064.546328
ACACGTG4050.063.150167
AGTAACA45000.062.683853
ACAAGAG47450.059.5819747
GTCGCCC2550.057.79001145
TGACGCC4450.052.038834145
AGCGTGC554.0106085E-450.4317678
Produced by FastQC (version 0.11.5)