FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_102_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_102_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424163
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC14100.075.457641
TAGTAAC79300.075.0992662
GTAGTAA87450.068.969341
AAACACG47250.068.419045
GTAACAA88600.066.5048144
ACACGAG35150.062.095527
AACACGA37200.059.786976
GCACGCA18650.058.9006232
AGTAACA103500.057.2676433
CACGAGC38600.055.6366358
ACGAGCC9900.054.6075559
AACACGT18800.054.3747756
GCCGACC7300.053.86234145
CGACGAA12150.053.165707145
TAACAAG111150.052.8874935
CTATCGA400.00687013652.660053145
TCTCGGG14550.047.2915145
ACAACGC19150.045.943228
CGCACTT20550.045.7151345
ACGAGCA27750.045.45719
Produced by FastQC (version 0.11.5)