FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n02_FLUB_100_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n02_FLUB_100_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT36290.327088989115661No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC35390.3189771100800012No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT26670.24038201542338603No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21070.1899081014237249No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20880.1881955936273078No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC15450.1392539234454936No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT15170.13673022774551052No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC14650.13204336430268485No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC14140.1274466328491443No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC14100.12708610489200384No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG14020.12636504897772297No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA13770.12411174924559525No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC13630.12284990139560371No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT12950.1167209261242163No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA12910.11636039816707586No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC12730.1147380223599439No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA12620.11374657047780772No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT12600.11356630649923749No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA12500.11266498660638638No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG12450.11221432665996084No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG12290.11077221483139911No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT11740.1058149554207181No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA11660.10509389950643722No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC11500.10365178767787549No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG11390.10266033579573927No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC11340.10220967584931373No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA11200.1009478279993222No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA11190.10085769601003709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA21250.0108.271974
CACGCAC2300.0102.14593
GCACGCA2400.097.903052
AGCACGC2450.095.9050451
GAAACAC7650.095.735974
TTCGGAA750.093.582756145
AAACACG7950.092.123295
TAACAAG25750.089.350655
GAGCAGA2400.086.385051
GACCCCC1350.083.18467145
CGCACTT3200.075.5698855
TCTCGGG1900.073.88112145
TAGTAAC31500.073.495862
ACGCACT3500.071.0665364
TAGAAAC10900.071.01012
AGAAGCG1950.070.8640755
AACACGT2150.070.6928946
AACACGA8250.068.6675346
ACACGAG8450.067.0422747
GCCGACC1600.065.80037145
Produced by FastQC (version 0.11.5)