FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9846353
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA15000.075.996982
TAGTAAC78150.075.030612
AAACACG29900.072.435655
TAACAAG83850.069.5874255
AACACGT11600.062.645836
CACGCAC19500.059.1875533
ACACGAG26350.054.61617
CGCACTT20350.052.86435
ACACGTG13900.052.2799727
ACGCACT23400.050.8452764
ACAACGC16600.041.2015088
GTAGTAA152600.040.316011
GTAACAA146450.040.2801364
AACAACG17800.039.2243737
AGAAGCG10550.036.4662635
CGGAGCG3300.034.542685
TCGAATA1204.4971312E-429.686322
AACAAGA210600.028.0443976
CACGAGC51300.027.9144238
GCAGAGC74450.026.3158843
Produced by FastQC (version 0.11.5)