FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_POSITIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_POSITIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952764
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT166670.5644541859762582No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT146600.4964839723052706No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC121330.41090314024419156No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT87430.29609545497032613No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT79610.26961179423753473No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT72220.24458439617930863No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT66260.22439991817835764No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT59550.20167544714037425No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC54290.1838616293073202No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT52930.17925577526683473No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG50750.17187286217252717No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA42740.14474573653702089No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT42450.14376360589603504No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG42200.14291694155035756No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT41340.14000441620122706No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG39150.13258763653309238No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT37990.1286591139691489No Hit
GTATTATTATCACTATATGTGTCAGCTTTATTATCATACTTACTATATTC36610.12398552678100924No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC36600.12395166020718217No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATACTAAGAACAC36080.12219059836817302No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT35510.12026020366002836No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT35240.1193458061666967No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT34560.11704287914645396No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC34030.11524795073361772No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33860.11467221897855705No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATTGTTCAAACA33530.11355462204226277No Hit
GGTCTGATTGCACTTGTGTTTGGTTTCAAAGCATCCATTCCCTATCTCTA33420.11318208973016468No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA33380.11304662343485627No Hit
GAACTAGCAGAAAAATTACACTGTTGGTTTGGTGGGAAAGAATTTGACCT33350.11294502371337499No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG33140.11223382566300591No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC32010.10840690282054373No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC31540.10681517385067009No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT31160.10552824404524033No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC31110.10535891117610482No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG30920.10471544627338994No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT30860.10451224683042735No Hit
CTATATGTGTCAGCTTTATTATCATACTTACTATATTCGGATATATTGCT30280.10254798554845562No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGCAAACAAG30160.10214158666253043No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30110.10197225379339493No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT30060.10180292092425944No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA29820.10099012315240906No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA29690.10054985769265679No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT29580.10017732538055868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCG100.007091457145.0007145
ATAGCGC100.007091816144.998256
ACTGTGG100.007091816144.998253
TACCGAG100.007091816144.998253
GTAACAA68600.0131.365024
AGCACGC23000.0129.636181
GAGCAGA3000.0128.164261
GCACGCA23800.0125.813692
CACGCAC23950.0125.019363
GAAACAC23650.0123.8462754
AAACACG25700.0113.967495
TAACAAG84800.0106.5258265
CGCACTT30150.0101.234275
TAGAAAC30800.0100.9860462
ACGCACT30500.0100.785674
CGGTGCA301.5996722E-596.67042
TAGTAAC97200.093.2392042
CAGCCTT2800.088.0346455
GCACTTA259.3890284E-486.998955
AGTAACA109400.082.969743
Produced by FastQC (version 0.11.5)