FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_PLASMID_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_PLASMID_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973843
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT83340.28024344257581857No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG79370.2668937129498766No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT77550.2607736857661955No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG67730.22775244019270688No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG54970.18484499686096406No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA53320.1792966205680663No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT52920.17795155964857595No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT52490.17650561916012378No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT50370.16937679628682484No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC47790.16070115335611193No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA46060.1548837648793161No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA44590.14994066600018896No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG39920.13423707976513893No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT38560.12966387263887164No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA38170.12835243824236856No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT37530.12620034077118397No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT36230.12182889278284026No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG36170.1216271336449167No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT35990.12102185623114603No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC34630.11644864910487877No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT33660.11318687637511461No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG33260.11184181545562426No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA32750.11012686278327403No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT32510.1093198262315798No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT32060.10780663269715314No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT30940.1040404621225801No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT30750.10340155818582218No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG30410.10225825640425537No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG30250.10172023203645923No Hit
ATATGTAATCATCGCCAAAAACATTCGAGGGTTTTGATTTTCATTCCACT30010.100913195484765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC400.00605264454.3736654
TAAGGAG400.00605264454.3736655
TTCGGAC1750.049.7130663
CTTCTAA27050.047.2001081
CGGACAT1850.047.025875
TCGGACA1850.047.025874
TAGATGG27200.046.910618
TACTGAT17250.046.6510287
CATATAC17450.046.1163443
ACTGATG17500.045.9845858
TTCTAAT28100.045.40892
GACAGGG803.723277E-545.3395921
GATGTGT657.339817E-444.614297
TATACTG38550.038.7409445
TTAGAAG34700.038.2339324
CCAGAGT1901.2914825E-1038.156963
ATTAGAA35150.037.9507033
AAGAGGC34850.037.861348
ATGTGTA800.00203750636.2491078
TGTGTAT800.00203750636.2491079
Produced by FastQC (version 0.11.5)