FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_NEGATIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_NEGATIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3693
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT1022.761982128350934No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT461.2455997833739507RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT421.1372867587327375RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG371.0018954779312212RNA PCR Primer, Index 27 (96% over 30bp)
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAG260.7040346601678852No Hit
GTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGCAGAA210.5686433793663688No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT210.5686433793663688RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA110.29786081776333606RNA PCR Primer, Index 27 (96% over 30bp)
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTCTT100.27078256160303277No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT100.27078256160303277No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG90.2437043054427295RNA PCR Primer, Index 27 (100% over 24bp)
CTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTTGTTACTAC90.2437043054427295No Hit
ATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTA70.18954779312212292No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTCC70.18954779312212292No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCTTATACACATCTCC70.18954779312212292No Hit
TGTTTCTACTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTT70.18954779312212292No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTC60.16246953696181965No Hit
GTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT60.16246953696181965No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCTGTCTCTTATACACATCTCCG60.16246953696181965No Hit
CCCCAAGTGCGTGCTTCTGCTCCCCCCGCTCTTGTTACTACCTGTCTCTT50.13539128080151638No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT50.13539128080151638No Hit
AGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTCCG50.13539128080151638No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT50.13539128080151638No Hit
ATTAGTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGC50.13539128080151638No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTACCTGTCTCTT50.13539128080151638No Hit
GTAGAAACACGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT50.13539128080151638No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTC50.13539128080151638No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC50.13539128080151638No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTGTCTCTTATAC50.13539128080151638No Hit
CTTGTTACTACTAATAACCCGGAGTAACAAGAGCCGGGTTATTAGTAGAA50.13539128080151638No Hit
CTTGTTACTACTAATAACCCGGAGTAACAAGAGCCCGGGTTATTAGTAGA50.13539128080151638No Hit
ATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACTGTCTCTTATA50.13539128080151638No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTC50.13539128080151638No Hit
GAGCAGAAGCACGCACGTGTTTCTACTAATAACCCGGGCTCTTGTTACTA50.13539128080151638No Hit
AGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAGTA40.1083130246412131No Hit
AACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG40.1083130246412131No Hit
TATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTT40.1083130246412131No Hit
ACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGAGCTCTTGTTAC40.1083130246412131No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCCTGTCTCTTATA40.1083130246412131No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCCTGCTTCTGCT40.1083130246412131No Hit
CTTCTGCTCCCCCCGCTCTGCTTCTGCTCTGTCTCTTATACACATCTCCG40.1083130246412131No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTGTCTCTTAT40.1083130246412131No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTC40.1083130246412131No Hit
GTAGAAACACGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTTATAC40.1083130246412131No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC40.1083130246412131No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC40.1083130246412131No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGT40.1083130246412131No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTGTCTC40.1083130246412131No Hit
GAGCAGAAGCGGAGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACT40.1083130246412131No Hit
AGCACGCACGTGTTTCTACTAATAACCCGGGCTCTTGTTACTACCTGTCT40.1083130246412131No Hit
CAGAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTC40.1083130246412131No Hit
GCAGAAGCACGCACGTGTTTCTACTAATAACCCGGTGCGTTGTTTCTACT40.1083130246412131No Hit
TATTAGTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAG40.1083130246412131No Hit
AGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAG40.1083130246412131No Hit
GTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGCAGAAGCA40.1083130246412131No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGT40.1083130246412131No Hit
ATTAGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGT40.1083130246412131No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGCTCCGCTTCTGC40.1083130246412131No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGT40.1083130246412131No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCT40.1083130246412131No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTCTT40.1083130246412131No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGCTGTCTCTTAT40.1083130246412131No Hit
AGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTAT40.1083130246412131No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGTG100.0068060136145.09
AAGAGCG100.0068060136145.09
CAAGAGC500.0116.08
AAGAGCC352.2459972E-7103.571439
TAGTAAC605.456968E-1296.6666642
GTAGTAA605.456968E-1296.6666641
AACAAGA659.094947E-1289.2307746
TAACAAG659.094947E-1289.2307745
ACAAGAG659.094947E-1289.2307747
GTAACAA659.094947E-1289.2307744
ACGTGCC258.6500146E-487.09
AGTAACA701.8189894E-1182.857143
AGAAGCG300.00178434572.55
ACGAGCC405.814343E-572.59
GAAACAC1005.456968E-1265.254
ACACGAG605.547916E-660.4166687
CACGAGC605.547916E-660.4166688
AACACGA605.547916E-660.4166686
TAGAAAC1101.2732926E-1159.3181842
GTAGAAA1101.2732926E-1159.3181841
Produced by FastQC (version 0.11.5)