FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_DEMUX_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_DEMUX_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT1133.503875968992248No Hit
GACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTAT501.550387596899225No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT421.302325581395349TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG391.2093023255813953TruSeq Adapter, Index 3 (96% over 28bp)
GTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACA320.992248062015504No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT300.9302325581395349RNA PCR Primer, Index 3 (95% over 23bp)
ACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGG260.8062015503875969No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC260.8062015503875969No Hit
GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCT190.5891472868217054No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT190.5891472868217054TruSeq Adapter, Index 3 (96% over 26bp)
ATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATC180.5581395348837209No Hit
GGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGAC180.5581395348837209No Hit
ATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG170.5271317829457364No Hit
GTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC170.5271317829457364No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA150.46511627906976744RNA PCR Primer, Index 3 (96% over 28bp)
AAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA150.46511627906976744No Hit
AACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG140.4341085271317829No Hit
GTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC140.4341085271317829No Hit
GCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG140.4341085271317829No Hit
GGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG130.40310077519379844No Hit
CCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCC130.40310077519379844No Hit
GAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT120.37209302325581395No Hit
GCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG120.37209302325581395No Hit
GAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCG120.37209302325581395No Hit
GTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACA120.37209302325581395No Hit
CCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGG110.34108527131782945No Hit
GTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACC110.34108527131782945No Hit
TTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTT90.27906976744186046No Hit
CCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGG90.27906976744186046No Hit
CCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGT80.248062015503876No Hit
AAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTA80.248062015503876No Hit
ATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGT80.248062015503876No Hit
GGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG70.21705426356589144No Hit
ATTAGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTG70.21705426356589144No Hit
GAGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACC70.21705426356589144No Hit
CGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGG70.21705426356589144No Hit
CGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCT70.21705426356589144No Hit
ACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGG70.21705426356589144No Hit
GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTC60.18604651162790697No Hit
TGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGA60.18604651162790697No Hit
GCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGG60.18604651162790697No Hit
AGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT60.18604651162790697No Hit
GACGACAACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAA50.15503875968992248No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG50.15503875968992248TruSeq Adapter, Index 3 (95% over 24bp)
TTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGT50.15503875968992248No Hit
GAGCAGAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACC50.15503875968992248No Hit
ACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGC50.15503875968992248No Hit
ATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGAC50.15503875968992248No Hit
GAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC50.15503875968992248No Hit
CCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCA40.124031007751938No Hit
AATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAAT40.124031007751938No Hit
ATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAAT40.124031007751938No Hit
ATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTATCCATGT40.124031007751938No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC40.124031007751938No Hit
AAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA40.124031007751938No Hit
CAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTA40.124031007751938No Hit
CCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAG40.124031007751938No Hit
AGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCC40.124031007751938No Hit
TACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTC40.124031007751938No Hit
TGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGAT40.124031007751938No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT40.124031007751938No Hit
GAGCAGAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACC40.124031007751938No Hit
TGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATAC40.124031007751938No Hit
ATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATT40.124031007751938No Hit
TCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTT40.124031007751938No Hit
AGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGT40.124031007751938No Hit
GCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTT40.124031007751938No Hit
GTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGAT40.124031007751938No Hit
ATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG40.124031007751938No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTCTT40.124031007751938No Hit
CTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGG40.124031007751938No Hit
GAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC40.124031007751938No Hit
AGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCC40.124031007751938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACA100.0067662164145.0145
AAAAAAA6057.4741165E-65.0330577125-129
Produced by FastQC (version 0.11.5)