FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_9_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_9_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282759
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC47040.36670956898372964No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT41760.32554829083249465No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC39340.3066827050131786No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT34050.26544346989574813No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT33190.25873917080293335No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC25540.19910209166336No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC23880.18616123527490355No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT23310.18171768820175888No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG21740.1694784445090621No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT21620.16854296091471585No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC21570.16815317608373825No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT21430.16706177855700097No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC21050.16409941384157117No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA20800.16215048968668316No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA20730.1616047909233145No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA19990.15583597542484598No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC19660.15326339554039378No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC19560.1524838258784386No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA18840.1468709243123611No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG17920.1396988834223732No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA17600.13720426050411652No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17440.13595694904498817No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17300.1348655515182509No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT16660.12987630568173758No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT16250.12668007006772122No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA16210.12636824220293913No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC16030.12496501681141976No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG15940.12426340411566007No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG15790.12309404962272727No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC15350.11966394311012435No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC15070.11748114805664976No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC14610.11389512761165582No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA14490.11295964401730957No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14000.10913975267372905No Hit
GCCCAAGCCATTGTTGCGAAGAATCCGTTTCCATTGGTAACGTTAGGGCA14000.10913975267372905No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA13680.10664512975547238No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA13560.10570964616112613No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA13500.105241904363953No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT13320.10383867897243364No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG13300.10368276504004259No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA13240.10321502324286946No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT13190.10282523841189187No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG12950.10095427122319936No Hit
AATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCT12860.10025265852743967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA27200.0117.5429844
AGCACGC1650.0109.918551
GAAACAC8100.0106.509674
AAACACG8450.0102.0980155
GCACGCA1800.0100.695832
TAACAAG31850.0100.609715
CACGCAC1950.092.946373
CGCACTT2100.082.855055
ACGCACT2450.079.8959354
TAGAAAC11400.079.4967042
GAGCAGA3250.075.894521
AACACGA9400.075.58326
AACACGT2050.074.266416
TAGTAAC44000.073.81922
CTGTCGT300.001930977272.546241
CGCACTG300.001936061872.498175
ACAAGAG45700.070.59457
ACGTGCA3500.070.4295352
GAAGCGG3650.067.577321
AGTAACA48800.066.55573
Produced by FastQC (version 0.11.5)