FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_99_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_99_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606493
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA33150.076.803071
TAGTAAC37950.066.4822542
AAACACG33700.065.8296365
AACACGA21800.064.184596
TAGACGA350.003567408362.141715
ACACGAG22850.060.9179087
CACGAGC23400.059.4860768
ACACGTG14900.056.441917
GTAACAA45550.054.4336784
ACGAGCT5600.053.0793729
ACGAGCA10400.052.979799
AGTAACA47350.052.823733
ACGAGCC10650.052.4168669
AACACGT15950.052.271766
AGCACGC6500.051.333331
GCACGCA7200.046.3194772
TAACAAG54150.046.0563935
GTAGAAA67650.044.6046871
CAACGCA12000.042.8950429
GAAGCGG11300.042.366291
Produced by FastQC (version 0.11.5)