FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_95_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_95_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188398
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA61650.1471923155344836No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG45910.10961231478001851No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA44620.10653237825058649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA32000.083.603142
TAGTAAC90500.083.5568162
GTAGTAA99650.077.458631
AGCACGC36350.073.642321
GTAACAA103250.072.813874
CGCACTT34300.069.538815
AAACACG64800.069.029645
CACGCAC39700.068.4803853
AGTAACA116000.065.2481163
ACGCACT45050.063.5664184
ACAACGC17100.062.322768
TAACAAG124450.060.5848275
ACACGAG49900.060.148567
AACACGA50400.059.9833836
ACGAGCC20150.055.407839
CACGAGC54950.054.0930678
GAAACAC83650.053.5609174
AACACGT27650.052.4397136
AACAACG20800.051.9335987
GTAGAAA120450.050.591551
Produced by FastQC (version 0.11.5)