FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_94_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_94_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678259
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT64000.38134757507631417No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC62540.37264808351988576No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT46470.2768940908405675No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT43760.26074640445842984No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT37000.22046656684099414No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT32560.19401057882007486No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC30090.17929294584447336No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG26950.1605830804422917No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23670.14103901721963058No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC22540.1343058490971894No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT21220.12644055536124044No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC20430.12173329623139217No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC20240.12060117061788436No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT20230.1205415850592787No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA19710.11744313601178365No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC19630.11696645154293825No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19620.11690686598433257No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG19520.11631101039827584No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT19180.11428510140568292No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT19170.11422551584707723No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG18980.11309339023356942No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT18680.11130582347539922No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA18600.11082913900655382No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC18410.10969701339304601No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC18190.10838613110372118No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA17840.10630063655252257No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT17840.10630063655252257No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT17400.10367887197387292No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17280.10296384527060483No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG17280.10296384527060483No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG16950.10099752183661759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA35900.0124.4003754
AGCACGC6650.0116.705591
GAAACAC13550.0115.036224
GCACGCA6800.0114.080212
CACGCAC6850.0112.189123
TAACAAG41550.0107.484325
AAACACG14550.0107.129955
TAGAAAC19250.089.6358262
TAGTAAC52750.085.35022
AACACGT3750.085.066166
CGCACTT9750.081.794385
AACACGA15250.081.29466
ACGCACT10000.080.474524
AGTAACA56400.079.826653
ACGTGCA6450.078.68172
ACACGAG16550.074.908927
CAGCATC1550.074.8382644
GAAGCGG4550.073.329041
ACAAGAG63150.070.94977
CGGAGCA7400.070.5401152
Produced by FastQC (version 0.11.5)