FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_92_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_92_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861699
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG15210.17651175178339534No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT13150.15260549217302097No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT13010.15098079491794697No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA12790.14842769923140214No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA12430.14424990628978332No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT12300.1427412588386432No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA11680.1355461709947441No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA10840.12579798746430018No Hit
CTGTAGGACATTTGTCCATTGAAGGCATCAAAGAAGCAGATATAACCCCA10220.1186028996204011No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT10170.1180226506007318No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA9710.11268435961977441No Hit
GTACATGATGGTGGGAAAACGACTTGGCACTCAGCAGCAACAGCCATTTA9450.10966706471749416No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC8930.10363247491293363No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA8640.1002670305988518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTT151.2300507E-4144.996926
ACAACGC2750.081.725548
ACGTGCC1650.074.695399
CAACGCA3050.073.686969
GCGTAGA406.663366E-572.498462
CACGAGC5700.067.410868
AAACACG9550.066.045725
GCACGCA1400.062.141542
AACACGA6450.061.8203936
TAGAAAC12700.056.514552
ACACGAG6950.056.3297427
CACGCAC1700.055.4400023
ACGAGCC4350.054.9988339
GAAACAC12000.053.165544
ATACCCG1258.913048E-1152.1988955
TAGTAAC8500.052.028312
GTAGTAA9550.048.5994571
ATTAGTA4350.048.3463361
CGCTATA450.00963507648.337917145
GTAGAAA17500.045.1692921
Produced by FastQC (version 0.11.5)