FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_91_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_91_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928752
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT66230.7131074818681413No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38130.4105509328647475No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT33730.3631755301738247No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23160.24936689234585765No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA23150.2492592209761056No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA21820.2349389287990766No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC21560.23213947318552206No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT21430.23073974537874484No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT14960.16107636914913778No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA13660.1470790910813651No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA12820.13803469602218893No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12750.13728099643392425No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG12380.13329715575309664No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11740.1264061880889624No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT11610.12500646028218512No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT11570.12457577480317673No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC11490.12371440384515994No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT11420.12296070425689527No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG11320.1218839905593743No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA11010.11854617809705928No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG10870.11703877892052991No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC10750.11574672248350475No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT10470.11273192413044603No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA10350.11143986769342085No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG10170.1095017830378831No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA9980.10745602701259324No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA9930.10691767016383276No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT9820.10573328509655969No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA9610.10347218633176565No Hit
AGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATTGTTCAAACA9440.10164177304597997No Hit
CTTTACATATAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG9420.10142643030647579No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG9300.10013437386945062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACA100.007077644145.089971
GGTTTGG100.007077644145.089971
CACCCGA151.2301045E-4144.996252
CGGGAAG100.0070913327144.996237
CGGTGCA100.0070913327144.996232
TAGTACG100.0070913327144.996234
AGCACGC9050.0131.462741
GCACGCA9250.0128.537222
CACGCAC9450.0126.584013
GAAACAC7350.0114.418794
AAACACG7500.0112.130425
ACCCGAA203.866369E-4108.747183
CCCGAAA203.866369E-4108.747184
GTAACAA24400.0105.181694
CGCACTT12050.0102.27955
ACGCACT12000.0102.101524
TAACAAG27700.092.912745
AACACGT2150.087.672146
ACACGTG2250.083.7756047
AACACGA8050.081.053796
Produced by FastQC (version 0.11.5)