FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_89_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_89_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486363
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT49380.3322203257212404No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT45360.3051744425823301No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC43200.29064232626888586No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27970.18817745059585042No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT24700.16617744117688613No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA23190.1560184154207283No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC22410.15077070675198453No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA20480.13778599171265699No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA18620.12527222488719109No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG18590.12507038993839326No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC17590.11834255831179867No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC16340.10993276877855544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA10950.0112.551642
AGCACGC10900.0111.854361
CACGCAC11950.0103.1330953
AAACACG13600.097.017385
CGCACTT13500.092.902925
ACGCACT14150.089.1476444
GAAACAC15550.084.851214
GTAACAA47000.083.910844
CGAGCCG353.438651E-582.853145
TAACAAG49350.079.9150855
TAGTAAC49700.079.644042
GAAGCGG4500.072.572181
CGGGTAC406.662514E-572.50382145
AGTAACA54800.072.3642043
GTAGTAA56850.070.593521
ACAACGC3450.065.1417858
AACACGA16000.062.9813356
AACACGT5000.062.3469926
GCCGAAC350.003567526862.1398547
ACGTGCA5700.061.0496832
Produced by FastQC (version 0.11.5)