FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_86_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_86_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561466
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG25910.16593380835701832No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC22500.14409535654314598No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT22160.14191791559982733No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT22010.1409572798895397No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC21250.13609005895741566No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC19380.12411413376916308No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA18580.11899074331429567No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA18420.11796606522332218No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG18350.11751776855852128No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT18340.11745372617783545No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT18220.11668521760960532No Hit
CTTTACATATAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGGG17950.11495607333108758No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17390.11136970001268039No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA17150.10983268287622017No Hit
TCCCAATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAG16840.10784736907495905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG256.467113E-6115.9960563
GCACGCA1600.090.6219252
AGCACGC1750.082.9074251
CACGCAC1950.074.356443
ACGTGCA1600.072.4975369
AAACACG8900.071.682955
GTAACAA24850.069.142524
GAAACAC9700.065.770964
CACGAGC6850.065.61828
ACAACGC2550.065.389938
AACACGA6900.065.142716
CAACGCA2700.064.4422459
AGAAGCG3800.061.0505565
ACGAGCC3000.060.414619
ACGAGCT651.09415105E-555.7673349
ACACGAG8200.055.699337
TAGTAAC31750.055.0296252
CACGTGC3600.054.373158
ACGTGCC1353.6379788E-1253.7018749
GAAGCGG6050.052.759271
Produced by FastQC (version 0.11.5)