FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_85_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_85_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563441
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG47780.18639009050725178No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA26370.10286954137036897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA59850.098.9032751
TAGTAAC62550.093.7746662
AGCACGC23750.090.103781
GCACGCA25750.083.344852
GTAACAA71750.081.340044
AGTAACA73200.080.0259253
TAACAAG81600.071.699115
CGCACTT27750.071.061825
AAACACG39500.069.194995
ACGCACT35600.067.6111764
CACGCAC34200.064.019353
AAGAGCG15450.057.2481889
ACAACGC12000.056.7906768
AACACGT16050.056.011486
CAAGAGC102400.054.6572468
ACGAGCG4000.054.374059
ACAAGAG107800.054.3404247
AACAAGA110750.052.6965986
GAAACAC51600.052.547534
GAAGCGG16200.050.599711
Produced by FastQC (version 0.11.5)