FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l02_n01_FLUB_84_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l02_n01_FLUB_84_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243702
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG18050.14513122918512633No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG16720.13443734913990651No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT15990.1285677758820039No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC13960.1122455379182473No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA13890.11168270212639363No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT13700.11015500497707649No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT12480.10034558117619816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG352.762281E-7103.570639
AACACGT1950.089.230086
GAAACAC6800.071.433274
AAACACG7000.068.3566065
ACGTGCC851.6916601E-1068.2347649
GTAACAA15600.064.598864
ACACGTG2700.064.443947
TAGTAAC17250.061.782132
CACGAGC4800.060.41628
TAACAAG17750.056.7742085
AGAAGCG3450.052.5358245
AACACGA5700.052.148726
ACGAGCA3700.050.945559
ACACGAG5750.050.4343957
GAAGCGG5650.050.0680161
CTACGCC2250.048.334904145
AGTAACA22500.047.36633
TAGAAAC14250.044.7715842
TAGTAGT3900.042.756083
AGCGGAG6450.042.7128453
Produced by FastQC (version 0.11.5)